Coding
Part:BBa_K1963006
Designed by: Frank Sargent Group: iGEM16_Dundee_Schools (2016-10-09)
A translational fusion between E. coli OsmY and Serratia Hfq
The E. coli OsmY protein (BBa_K892008) is secreted from the cell, and this has been characterised by at least two iGEM teams and other scientists in the field (e.g. BBa_K1963007). Serratia Hfq is an RNA binding protein (see BBa_K1963001). This translational fusion between the two may allow Hfq to be secreted from an E. coli chassis organism, together with any specific sRNA that may be attached. Pairing Serratia Hfq and Serratia sRNA sequences may afford some specificity to the system.
Usage and Biology
This biobrick was used as the basis of a rhamonose-regulated composite part BBa_K1963009.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//awards/part_collection/2016
None |